Fingerprint Folding Fun

A busy summer of 2022 for our Leeds Fellow Sam Confederat with publication of his impressive  paper in Biophysical Journal. In this study Sam uses DNA origami, a technique through which various DNA constructs can be designed, ranging from single tiles to the self-assembly of large-scale, complex, multi-tile arrays.

Solid-state nanopores have enabled the single-molecule detection of a range of analytes but often lack the ability to provide quantitative information of heterogeneous samples. Here, Sam and his collaborators at Leeds have been able to demonstrate a single-molecule and high-throughput approach for the analysis of higher-order DNA origami assemblies with a nanopore. The technique enables the characterization of DNA origami nanostructures at statistically relevant numbers in real time and at single-molecule resolution while being non-destructive and label-free and without the requirement of lengthy sample preparations or the use of expensive reagents. It has enabled the authors to quantify the assembly yield of DNA origami nanostructures based on their equivalent charge surplus computed from the ion current signals recorded.

An excellent contribution, Sam!